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Introduction to Preparation of Culture Media of Microbiology

The culture media of microbiology is a mixed nutrient for the growth, reproduction and metabolism of microorganisms. Because microorganisms have different types of nutrition, different requirements for nutrients, and different research purposes, there are many kinds of culture media and different raw materials. But in terms of nutrition, bacterial growth media generally contains carbon source, nitrogen source, inorganic salt, auxin, water and so on. In addition, culture media of microbiology has appropriate PH Value, buffer capacity, redox potential and osmotic pressure. In order to meet the specific requirements of research, the preparation and sterilization of culture medium are needed.


Attention should be paid to:


When preparing solid media, we first need to boil the prepared liquid medium, and then add the qualified agar, and continue heating until completely melted, then stir constantly to avoid burning the agar paste bottom.


The filtered media should be repackaged.


After repacking, the mouth of a pipe or bottle needs to be plugged with a cotton plug.


After medium sterilization, if you want to make a slant medium and flat medium, the process should be done when the medium is not set.


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