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Microbiology Test

Main Techniques in Food Microbiology Testing

Morphological inspection technique for food microbiology testing

The culture medium is a nutrient substrate that is artificially prepared to suit the growth, reproduction, or accumulation of metabolic products of microorganisms. Any culture medium must contain the necessary energy, carbon source, nitrogen source, mineral elements, water, and growth factors required by microorganisms, but different nutritional types and different species of microorganisms have very different requirements for nutritional elements. Microorganisms in food can be identified by colony morphology observation after selective culture on culture media to determine whether they are fungi or bacteria, and then bacteria can be further identified by inspection of unstained bacterial morphology specimen or stained bacterial specimen staining method to determine the type of contaminating bacteria, such as Vibrio, Pseudomonas aeruginosa, Staphylococcus aureus, etc. This testing technique is still widely used in food microbiology testing methods, but it has the disadvantages of being time-consuming and complicated to operate.

Genomic testing technique for food microbiology testing

With the development of genomic testing techniques, PCR technology has been widely used in the detection of food microorganisms. Gene chip technology based on PCR is also gradually being applied to food microbiology exam. PCR technology refers to the application of polymerase chain reaction in molecular biology, which can amplify specific DNA fragments of extremely small amounts to millions of times or more. PCR technology has the advantages of fast program, simple operation, high sensitivity, and high specificity. In food microbiology exam, PCR can be used to quickly determine whether a particular microorganism is present by amplifying the target DNA of the microorganism under detection. Moreover, PCR can accurately detect some microorganisms carried in food that cannot be cultured artificially or are difficult to culture, thereby increasing the diagnostic check content and diagnostic ability during the microbiology test in food industry.

Real-time detection system for food microbiology testing

A long-time dream of food microbiologists has been to use a single method to collect, process, and analyze various pathogenic microorganisms in food. Real-time monitoring of microorganisms is a microbiology lab kit and means that can basically meet this requirement. The real-time monitoring system for microorganisms can detect food samples of different forms including solid, liquid, surface, paste, etc., with simple operation, small amount of test food, and no pre-processing required, and can obtain high sensitivity target microorganism detection results. Through real-time monitoring system for food microbiology testing, quantitative and qualitative detection of various routine microorganisms and pathogenic microorganisms can be quickly completed, thereby controlling food microbiological content to ensure the hygiene and safety of food.

In the development of the new era, our material life is constantly improving. In terms of food, we no longer just focus on the issue of food and clothing, but modern people pay more attention to health and wellness issues in food. Food safety indicators are highly valued, and in order to improve the food microbiological safety index, food microbiology exam must continue to increase supervision and corresponding improvement of the level of microbiological testing technology.

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