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Microbiology Test

Reasons for Applying Sterile Homogenizers in Food Industry

Principle and Application of Sterile Homogenizer

A sterile homogenizer, also known as a tapping homogenizer, is used to process solid samples and extract bacterial components by placing the original sample and a liquid or solvent into a homogenization bag. The machine repeatedly strikes the sample bag's hammer plate, generating pressure and causing vibration to accelerate mixing, resulting in a uniform distribution of microbial components in the solution. The sterile homogenizer is mainly used in the process of extracting bacteria from solid samples. It only requires adding the sample and diluent solution into a sterile sample bag and placing it in the tapping homogenizer to complete the sample processing, effectively separating the microbial content inside and on the surface of the solid sample, ensuring that all sample mixtures are mixed in the sterile bag. After processing, the sample solution can be directly sampled and analyzed without the danger of changes to the sample or cross-contamination.

The sterile homogenizer is suitable for homogenizing animal tissues such as brain, kidney, liver, spleen, and microorganisms, as well as cosmetic products. It is particularly suitable for the preparation of microorganism detection samples as it is non-contaminating, non-destructive, does not require sterilization, and does not need to clean vessels. During homogenization, lipase is activated, the surface area of the fat globules increases, and the hydrolysis of fat accelerates. The impact on proteins manifests as a reduction in the size of casein micelles and the transfer of some proteins from the serum to the fat globules, supplementing and increasing the surface area of the fat globules. Due to preheating and high pressure before homogenization, some proteins are denatured, which lowers their stability. Homogenizing milk with a sterile homogenizer produces a softer curd texture, high water-holding capacity, and smaller fat globules and enzyme protein micelles, which increases the digestive rate, absorption rate, and protein utilization rate. It has been widely used in microbiology test in food industry and bacterial detection fields.

Application of Sterile Homogenizer in the Food Industry

The sterile homogenizer makes the process of extracting bacteria from solid samples very simple. The food homogenizer only needs to add the sample and diluent solution into sterile filter food sample bags, place the sample bag in the homogenizer, close the door, and start and complete the sample processing. It effectively separates the microbial content inside and on the surface of the solid sample in the sterile bag, ensuring that all sample mixtures have sufficient representativeness. It reduces the processing and preparation time of the samples, and processing time of 30-60 seconds is generally sufficient.

The system provides various standard modes, including almost all areas of analysis and research. The food homogenizer is equipped with a hammer plate adjustment device (the hitting device can adjust the distance back and forth) to ensure that samples of different processing volumes achieve homogenization effects. For the operator's safety, an automatic stop hall switch device is designed to prevent accidental clamping of fingers. The sterile disposable filter bag ensures hygiene and safety, and the sample does not come into contact with the homogenizer, eliminating the need for system cleaning.

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