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Microbiology Test

Introduction to the Application Scope, Working Principle, and Usage of Sterile Homogenizer

Scope of Application of Sterile Homogenizer

Sterile homogenizers are widely used in animal tissue, biological sample homogenization, and other processing applications. They are suitable for use in the food, pharmaceutical, cosmetic, clinical, molecular biology, toxin and bacteria detection fields. They are especially suitable for the preparation of microbiological testing samples.

The device uses a stainless steel system to effectively separate the outer surface of the protected sample and the uniformly mixed microorganism-containing sample inside. The sample is placed in a disposable sterile homogenization bag, avoiding contact with the instrument. It has the characteristics of gentle homogenization, contamination-free samples, no damage, no heating, no need for sterilization treatment, and no need to wash utensils. The device caters to the demands of rapid, accurate results, and high repeatability, making it an excellent option for microbiological testing of food samples and others.  This product is also suitable for the homogenization of tumor tissues (such as liver cancer, colorectal cancer, gastric cancer, and breast cancer), which can obtain a large number of single cells (reaching 2×105 within 2 minutes). If necessary, soft fragmentation of tissue cells (such as liver) can be achieved by extending the homogenization time.

Working Principle of Sterile Homogenizer

The working principle of sterile homogenizer is to form a strong, back-and-forth liquid shear, friction, centrifugal extrusion, liquid flow collision, and other comprehensive effects in the narrow gap between the stator and rotor. The sample is first mixed with a sterile diluent, which helps to dilute and suspend the sample uniformly. The homogenizer then utilizes high shear forces, which subjects samples to rapid and repeated pressure changes, leading to complete sample lysis. The material circulates back and forth in the container through the above working process to finally obtain the product.

Instructions for the Use of Sterile Homogenizer:

  1. Prepare the pretreated sample and place it in a sterile homogenization bag.

  2. Open the door of the homogenizer (fully openable), clip the opening of the sterile homogenization bag while closing the door.

  3. Select the program (such as tapping speed, tapping time, etc.) and start the homogenization process. After homogenization is complete, open the door of the sterile homogenizer, take out the processed sample, and temporarily place it on the sample rack.

Cleaning and Maintenance of Sterile Homogenizer

The design of sterile homogenizer ensures that the splashed sample treatment solution is retained in the treatment chamber. The power must be disconnected during the cleaning process.

When cleaning the external surface of the processor and oscillator, first use gentle soapy water to clean, and then use a soft cloth to gently dry (if the interior of the processor is contaminated with bacteria, use a 70% isopropyl alcohol solution for treatment). Do not use acid, alkali, chlorine solution, salt solution, or organic solvents to clean the external of the processor. Avoid using flushing and scraping methods during the cleaning process. Otherwise, irreversible damage to the processor may occur. If the cleaning method used is different from the one mentioned above, consult the manufacturer or agent to confirm whether the method used will damage the instrument.

The microswitch on the treatment chamber door of the sample processor can ensure that the instrument cannot operate if the sample entrance door is not properly closed. Do not interfere with the normal operation of the microswitch. If there is an increase or change in operating noise, report it promptly to the manufacturer or supplier for the necessary handling.

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